Inactivation of the Cholinesterase Gene by Alu Insertion: Possible Mechanism for Human Gene Transposition

• Published in: Proceedings of the National Academy of Sciences - PNAS 1991-12, Vol.88 (24), p.11315-11319
• Main Authors: MURATANI, K, HADA, T, YAMAMOTO, Y, KANEKO, T, SHIGETO, Y, OHUE, T, FURUYAMA, J, HIGASHINO, K
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - deficiency; Acetylcholinesterase - genetics; Alleles; Base Sequence; Biological and medical sciences; Cell lines; Chromosomes, Human, Pair 3; Cloning, Molecular; Complementary DNA; Consensus sequence; DNA; DNA Probes; DNA Transposable Elements; Exons; Female; Fundamental and applied biological sciences. Psychology; Genes; Genetic Linkage; Genetic mutation; Genomics; Humans; Introns; Male; Middle Aged; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutation; Nucleotides; Oligodeoxyribonucleotides; Pedigree; Polymerase Chain Reaction; Restriction Mapping
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.24.11315

The human cholinesterase (ChE) gene from a patient with acholinesterasemia was cloned and analyzed. By using ChE cDNA as a probe, four independent clones were isolated from a genomic library constructed from the patient's DNA. Sequencing analysis of all of the four clones revealed that exon 2 of the ChE gene was disrupted by a 342-base-pair (bp) insertion of Alu element, including a poly(A) tract of 38 bp, which showed 93% sequence homology with a current type of human Alu consensus sequence. Southern blot analysis showed that the Alu insertion occurred in both alleles of the patient and was inherited in the patient's family. This Alu insertion was flanked by 15-bp of target site duplication in exon 2 corresponding to positions 1062-1076 of ChE cDNA, indicating that an Alu element could have been integrated by retrotransposition. Thus, this case provides an important clue to the mechanism of inactivation of a gene by integration of a retrotransposon.