CD43 Interferes with T-Lymphocyte Adhesion

• Published in: Proceedings of the National Academy of Sciences - PNAS 1992-06, Vol.89 (11), p.5001-5005
• Main Authors: Ardman, Blair, Sikorski, Maria A., Staunton, Donald E.
• Format: Article
• Language: English
• Subjects: Antibodies; Antigens, CD; Biochemistry; Biological and medical sciences; Cell Adhesion; Cell adhesion molecules; Cell Adhesion Molecules - physiology; Cell lines; Cellular biology; Endothelial cells; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HeLa Cells; Humans; Immunity (Disease); Immunobiology; In Vitro Techniques; Leukocytes; Leukosialin; Lymphocyte Activation; Lymphocyte Function-Associated Antigen-1 - metabolism; Lymphocytes; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Monoclonal antibodies; Neuraminidase - pharmacology; Sialoglycoproteins - physiology; T lymphocytes; T-Lymphocytes - cytology; Transfection
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.11.5001

CD43 is a cell-surface sialoglycoprotein of uncertain physiologic function expressed to various degrees by most leukocytes. We tested whether or not CD43 participates in intercellular adhesion by comparing the binding of human T lymphocytes to transfected HeLa cells stably expressing CD43 and sham-transfected HeLa cells (CD43-negative). Significantly fewer T lymphocytes adhered to the CD43-positive HeLa cells than to the CD43-negative HeLa cells. Diminished T-cell adherence to the CD43-positive HeLa cells was seen for all T lymphocytes tested, irrespective of their source or derivation. Antibody-blocking experiments revealed that CD43 interference with T-cell adhesion largely represented interference with T-cell leukocyte function-associated antigen 1 binding to HeLa cell intercellular adhesion molecule 1. The CD43 anti-adhesion effect was not overcome by treating cells with phorbol 12-myristate 13-acetate, a chemical that increases the binding avidity of leukocyte function-associated antigen 1 for intercellular adhesion molecule 1. However, neuraminidase treatment of the HeLa cell transfectants diminished the CD43 anti-adhesion effect. These data indicate that CD43 expression by opposing cells can interfere with cell-cell adhesion. The data also suggest that CD43 might regulate T-cell adhesion by interfering with leukocyte function-associated 1 binding to intercellular adhesion molecule 1, a major activation-induced adhesion pathway among lymphocytes.