Studies on the growth in culture of plant cells. XVIII. Nuclear cytology of Acer pseudoplatanus suspension cultures

Established suspension culture strains of Acer pseudoplatanus were analysed by chromosome counting and microdensitometric DNA measurements of individual nuclei. Comparison with the root-tip complement (2n = 4x = 52) showed the cultures to be entirely aneuploid with modal chromosome numbers of approx...

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Bibliographic Details
Published in:Journal of experimental botany 1974-08, Vol.25 (87), p.772-783
Main Authors: Bayliss, M.W, Gould, A.R
Format: Article
Language:English
Subjects:
Aneuploidy
Cell culture techniques
Cell cycle
Cell growth
Cell nucleus
Chromosomes
Cultured cells
plant anatomy
Plant cells
plant morphology
Ploidies
Steepest descent method
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Summary:Established suspension culture strains of Acer pseudoplatanus were analysed by chromosome counting and microdensitometric DNA measurements of individual nuclei. Comparison with the root-tip complement (2n = 4x = 52) showed the cultures to be entirely aneuploid with modal chromosome numbers of approximately 75 and 135 plus some cells with 250-350. Analysis of the frequency distributions of nuclear DNA values through the growth cycle of a batch culture showed that stationary phase cells accumulated in G1 of the cell cycle. Stationary phase DNA distributions could thus be used to indicate the chromosomal status of a culture without the complication of G1 and G2 DNA values for each chromosome number. Root-tip and cultured cells showed a close correlation between DNA content and chromosome number indicating that structural changes and loss of chromosomes had been at random.
ISSN:0022-0957
1460-2431