Temporally and Spectrally Resolved Subpicosecond Energy Transfer within the Peripheral Antenna Complex (LH2) and from LH2 to the Core Antenna Complex in Photosynthetic Purple Bacteria

• Published in: Proceedings of the National Academy of Sciences - PNAS 1995-12, Vol.92 (26), p.12333-12337
• Main Authors: Hess, S, Chachisvilis, M, Timpmann, K, Jones, M R, Fowler, G J, Hunter, C N, Sundström, V
• Format: Article
• Language: English
• Subjects: Absorption spectra; Antennas; Bacteriology; Energy transfer; Kinetics; Photosynthesis; Pigments; Room temperature; Spectral bands; Time constants; Wave excitation; Wavelengths
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.92.26.12333

We report studies of energy transfer from the 800-nm absorbing pigment (B800) to the 850-nm absorbing pigment (B850) of the LH2 peripheral antenna complex and from LH2 to the core antenna complex (LH1) in Rhodobacter (Rb.) sphaeroides. The B800 to B850 process was studied in membranes from a LH2-reaction center (no LH1) mutant of Rb. sphaeroides and the LH2 to LH1 transfer was studied in both the wild-type species and in LH2 mutants with blue-shifted B850. The measurements were performed by using ≈100-fs pulses to probe the formation of acceptor excitations in a two-color pump-probe measurement. Our experiments reveal a B800 to B850 transfer time of ≈0.7 ps at 296 K and energy transfer from LH2 to LH1 is characterized by a time constant of ≈3 ps at 296 K and ≈5 ps at 77 K. In the blue-shifted B850 mutants, the transfer time from B850 to LH1 becomes gradually longer with increasing blue-shift of the B850 band as a result of the decreasing spectral overlap between the antennae. The results have been used to produce a model for the association between the ring-like structures that are characteristic of both the LH2 and LH1 antennae.