Protein kinase LKB1 regulates polarized dendrite formation of adult hippocampal newborn neurons

Adult-born granule cells in the dentate gyrus of the rodent hippocampus are important for memory formation and mood regulation, but the cellular mechanism underlying their polarized development, a process critical for their incorporation into functional circuits, remains unknown. We found that delet...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2014-01, Vol.111 (1), p.469-474
Main Authors: Huang, Wei, She, Liang, Chang, Xing-ya, Yang, Rong-rong, Wang, Liang, Ji, Hong-bin, Jiao, Jian-wei, Poo, Mu-ming
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Autoantigens - metabolism
Axons
Axons - metabolism
Biological Sciences
Brain
Carrier Proteins - metabolism
Cell Polarity
Cell Proliferation
Cells
Correlation analysis
Dendrites
Dendrites - metabolism
Dentate Gyrus - metabolism
Gene Expression Regulation
Golgi apparatus
Golgi Apparatus - metabolism
Green Fluorescent Proteins - metabolism
Hippocampus - cytology
Hippocampus - metabolism
Kinases
Membrane Proteins - metabolism
Memory
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy, Confocal
Morphogenesis
Neurogenesis
Neurons
Neurons - metabolism
Phosphorylation
Physiological regulation
Protein-Serine-Threonine Kinases - metabolism
Pyramidal cells
Retroviridae
Rodents
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Summary:Adult-born granule cells in the dentate gyrus of the rodent hippocampus are important for memory formation and mood regulation, but the cellular mechanism underlying their polarized development, a process critical for their incorporation into functional circuits, remains unknown. We found that deletion of the serine-threonine protein kinase LKB1 or overexpression of dominant-negative LKB1 reduced the polarized initiation of the primary dendrite from the soma and disrupted its oriented growth toward the molecular layer. This abnormality correlated with the dispersion of Golgi apparatus that normally accumulated at the base and within the initial segment of the primary dendrite, and was mimicked by disrupting Golgi organization via altering the expression of Golgi structural proteins GM130 or GRASP65. Thus, besides its known function in axon formation in embryonic pyramidal neurons, LKB1 plays an additional role in regulating polarized dendrite morphogenesis in adult-born granule cells in the hippocampus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1321454111