Leaf Unrolling of Three Wheat Genotypes in Russian Wheat Aphid Extracts

The Russian wheat aphid (RWA) is an important pest on wheat and barley in several areas of the world. It would be desirable to have an indirect screening test for resistance when aphids are not available. Three wheat genotypes, one susceptible Carson and two resistant, CORWA1 and PI294994, to the Ru...

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Bibliographic Details
Published in:Cereal research communications 1994-01, Vol.22 (4), p.375-379
Main Authors: Dong, H., Quick, J.S., Brigham, D.L., Bjostad, L.B., Rudolph, J.B., Peairs, F.B.
Format: Article
Language:English
Subjects:
Bioassay
Chemical ecology
Coleoptiles
Genotypes
Grasses
Pathology
Plants
Screening tests
Seedlings
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Summary:The Russian wheat aphid (RWA) is an important pest on wheat and barley in several areas of the world. It would be desirable to have an indirect screening test for resistance when aphids are not available. Three wheat genotypes, one susceptible Carson and two resistant, CORWA1 and PI294994, to the Russian wheat aphid, were used to test their in vitro responses to aphid extracts. Etiolated seedlings at single leaf stage about 10 to 15 cm tall were cut into 1.5 to 2 cm long segments. The segments just above the coleoptile were incubated in crude RWA extract or buffer. The degree of unrolling of the leaf segments was scored. Significant differences existed in unrolling between the leaf segments incubated in buffer only and buffer plus RWA extract. There were also significant differences between the susceptible genotype and the two resistant ones. No significant difference was detected between the two resistant genotypes in leaf unrolling. This Laboratory leaf unrolling test shows promise for genotype selection and needs further testing.
ISSN:0133-3720
1788-9170