Osmotic stress in wheat seedlings: effects on fatty acid composition and phospholipid turnover in coleoptiles

Fatty acid (FA) and phospholipid (PL) composition, as well as phosphorus turnover in phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) from coleoptiles collected from Triticum durum, cv. Balcarceño-INTA seedlings under osmotic stress, have been studied. Wheat seedlings were water-stressed...

Full description

Saved in:
Bibliographic Details
Published in:Cereal research communications 1996-01, Vol.24 (1), p.77-84
Main Authors: Sueldo, R. J., Invernati, A., Plaza, S. G., Barassi, C. A.
Format: Article
Language:English
Subjects:
Coleoptiles
Dehydration
Fatty acids
Leaves
Lipids
Phospholipids
Phosphorus
Physiology
Plants
Radioactive decay
Seedlings
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fatty acid (FA) and phospholipid (PL) composition, as well as phosphorus turnover in phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) from coleoptiles collected from Triticum durum, cv. Balcarceño-INTA seedlings under osmotic stress, have been studied. Wheat seedlings were water-stressed in darkness with 20% polyethylene glycol (PEG) 6000 added to the root medium. At zero time and after a 36 h-treatment period, total lipid extracts (TLE) were obtained from coleoptiles. Gas liquid and thin layer chromatographic analyses of TLE revealed the presence of 6 FA and 11 PL classes. Double bond index (DBI) rose during coleoptile growth, being the rise less pronounced in water-stressed seedlings. The relative PL distribution did not change during treatment. However, coleoptiles from 32P-labelled seedlings grown in water at the dark increased their specific radioactivities in PC, while under osmotic stress a significant (P < 0.025) fall was evident. Here we show that lipid unsaturation and PC turnover in coleoptiles collected from wheat seedlings grown in darkness, are affected by osmotic stress.
ISSN:0133-3720
1788-9170