SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR SCREENING AFLATOXIN B 1 IN FOOD-AND FEEDSTUFFS

Aflatoxins are toxic metabolites produced by strains of Aspergillus flavus and Aspergillus parasiticus. They occur worldwide and contaminate certain foods and feedstuffs, especially wheat, corn, soya and groundnut products. Although the climate in Hungary is not favourable for production of these mo...

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Bibliographic Details
Published in:Cereal research communications 1997-01, Vol.25 (3), p.291-292
Main Authors: Barna-Vetró, Ildikó, Solti, L., Szabó, E., Gyöngyösi-Horváth, Á.
Format: Article
Language:English
Subjects:
Aflatoxins
Antibodies
Corn
Enzyme linked immunosorbent assay
Food
Fusarium
Monoclonal antibodies
Mycotoxins
Oral presentations
Toxins
Toxoid vaccines
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Summary:Aflatoxins are toxic metabolites produced by strains of Aspergillus flavus and Aspergillus parasiticus. They occur worldwide and contaminate certain foods and feedstuffs, especially wheat, corn, soya and groundnut products. Although the climate in Hungary is not favourable for production of these moulds, by consumption of imported foods these mycotoxins can cause serious health problems. As aflatoxin B1 is highly carcinogenic, its accepted level is regulated in many countries, the official limit in Hungary is 5 ng/g. Five years ago our institute started a research program for developing ELISA tests to detect mycotoxins in food and feeds, resulted in reagent-kits for T-2, zearalenone and ochratoxin-A. This program continued by development of sensitive, monoclonal antibody-based direct, competitive ELISA test and reagent kit for quantitative measurement of aflatoxin B1 with detection limit of 1.5 ng/g in cereals. The mean within-assay and interassay coefficients of variation of the standard curves were less than 10 %. With acetonitrile based extraction solvent the mean recovery values from cereals contaminated artificially with this mycotoxin was about 80 %. This test was offered for screening of samples in control laboratories.
ISSN:0133-3720
1788-9170