A simple and rapid molecular method for distinguishing between races of Fusarium oxysporum f.sp. ciceris from India

• Published in: Current science (Bangalore) 2001-02, Vol.80 (4), p.571-575
• Main Authors: Chakrabarti, Apratim, Mukherjee, Prasun K., Sherkhane, Pramod D., Bhagwat, Anjali S., Murthy, Narra B. K.
• Format: Article
• Language: English
• Subjects: Chickpeas; DNA probes; Ethanol; Fusarium; Gels; Genetic variation; Intergenic DNA; Mycelium; Pathogens; RESEARCH COMMUNICATIONS; Ribosomal DNA
• ISSN: 0011-3891

EcoRI restriction pattern of the nuclear ribosomal DNA from four isolates of Fusarium oxysporum f.sp. ciceris (FOC) representing four races prevalent in India indicated that the races could be grouped into three distinct groups; races 1 and 4 representing one group and race 2 and race 3, the other two. The restriction pattern indicated presence of three EcoRI sites on the nuclear rDNA of this species, one each on the 5.8S and the 25S regions, conserved to all, and the other one, i.e. the variable site, on the intergenic spacer (IGS) region of the nuclear rDNA. The same was confirmed by PCR-amplification of the IGS region followed by digestion with EcoRI and a set of other enzymes. It is suggested that amplification of the IGS region and digestion with restriction enzymes could be used to study polymorphism in FOC, and to rapidly identify the races existing in India. We also propose that out of the four types of races described from India, races 1 and 4 are the same.