In vitro induction and enlargement of apical domes and formation of multiple shoots in finger millet, Eleusine coracana (L.) Gaertn and crowfoot grass, Eleusine indica (L.) Gaertn

In cereal crops, somatic embryogenesis is the most common pathway of plant regeneration. We describe a protocol for high frequency regeneration of Eleusine coracana via formation of large apical domes from seeds, mature embryos, immature inflorescences and immature embryos. The induction of apical d...

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Bibliographic Details
Published in:Current science (Bangalore) 2001-12, Vol.81 (11), p.1482-1485
Main Authors: Kumar, Satish, Agarwal, Kalpana, Kothari, S. L.
Format: Article
Language:English
Subjects:
Callus
Embryos
Grasses
Immatures
Inflorescences
Millet
Nodules
Plantlets
Plants
RESEARCH COMMUNICATIONS
Seeds
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Summary:In cereal crops, somatic embryogenesis is the most common pathway of plant regeneration. We describe a protocol for high frequency regeneration of Eleusine coracana via formation of large apical domes from seeds, mature embryos, immature inflorescences and immature embryos. The induction of apical domes occurred on MS medium supplemented with different auxins [2,4-dichlorophenoxyacetic acid (2,4-D); 2,4,5-trichlorophenoxyacetic acid (2,4,5-T); and parachlorophenoxyacetic acid (pCPA)] and cytokinins [kinetin (Kn) and 6-benzylaminopurine (BAP)]. The primary domes, after four weeks of subculturing on MS + 2,4-D (0.1, 0.2 mg/l), proliferated rapidly and gave rise to secondary and tertiary domes along with green, nodular, compact callus. The domes on MS medium containing GA3; (1 mg/l) or 1-naphthaleneacetic acid (NAA) (1 mg/l) gave rise to high-frequency shoot bud differentiation. Multiple shoot buds arose over the entire surface of the dome, exogenously. Histological observations provided a clear evidence that the shoot apices of the mature and immature embryos transformed into enlarged apical domes and the surface of the domes was occupied by highly meristematic cells which differentiated into shoot buds. De novo differentiation of meristematic giant domes and shoot buds was also observed in the repeatedly subcultured callus raised from mature embryos as well as inflorescence. Similar structures were also seen in cultures raised from immature inflorescence of crowfoot grass (Eleusine indica).
ISSN:0011-3891