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Level of endogenous free amino acids during various stages of culture of Vigna mungo (L.) Hepper – Somatic embryogenesis, organogenesis and plant regeneration

A comparative study on endogenous free amino acid levels was conducted in embryogenic and regenerating tissues vis-à-vis regenerated shoots of Vigna mungo PS1. A number of important amino acids were found to increase during initiation of embryogenesis and regeneration. To study the effects of exogen...

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Published in:Current science (Bangalore) 2002-02, Vol.82 (4), p.429-433
Main Authors: Sen, Jayanti, Kalia, Sanjeev, Guha-Mukherjee, Sipra
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Kalia, Sanjeev
Guha-Mukherjee, Sipra
description A comparative study on endogenous free amino acid levels was conducted in embryogenic and regenerating tissues vis-à-vis regenerated shoots of Vigna mungo PS1. A number of important amino acids were found to increase during initiation of embryogenesis and regeneration. To study the effects of exogenous application of amino acids in this system, media were supplemented with three amino acids, tryptophan (Trp), serine (Ser) and proline (Pro). We found that in the presence of proline, a stress-associated amino acid, there was an enhancement in shoot-bud regeneration as well as in the production of somatic embryos. We also analysed the endogenous levels of amino acids in these tissues. This communication thus deals with the induction of globular embryo by auxin and its promotion by proline in V. mungo.
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subjects Amino acids
Auxins
Callus
Cotyledons
Embryos
Germination
Plant cells
Plants
RESEARCH COMMUNICATIONS
Somatic embryogenesis
Somatic embryos
title Level of endogenous free amino acids during various stages of culture of Vigna mungo (L.) Hepper – Somatic embryogenesis, organogenesis and plant regeneration
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