PRECIPITIN REACTIVITY OF SUPERNATANT FRACTIONS

Mild acid hydrolysis of yeast phase Histoplasma capsulatum cell walls yielded precipitin-reactive material in the supernates of hydrolysis. Three distinct bands were present at various times by means of double diffusion in agar gel employing rabbit anti-H. capsulatum antiserum. Band I was acid-labil...

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Bibliographic Details
Published in:Florida scientist 1978-10, Vol.41 (4), p.225-229
Main Authors: Sweeney, Michael J., White, Roseann S., Calcagno, John V., Oels, Helen C.
Format: Article
Language:English
Subjects:
Acid hydrolysis
Antibodies
ANTIGENIC FRACTIONS OBTAINED FROM MILD ACID HYDROLYSIS OF YEAST PHASE HISTOPLASMA CAPSULATUM CELL WALLS, I-III
Antiserum
Cell walls
Gels
Hydrolysis
Immunodiffusion
Precipitins
Reactivity
Yeasts
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Summary:Mild acid hydrolysis of yeast phase Histoplasma capsulatum cell walls yielded precipitin-reactive material in the supernates of hydrolysis. Three distinct bands were present at various times by means of double diffusion in agar gel employing rabbit anti-H. capsulatum antiserum. Band I was acid-labile, while bands 2 and 3 were acid-stable. All 3 bands were removed from the walls early in the hydrolysis. Precipitin-reactive material was found to be different from fluorescent and hemagglutinating antibody-reactive material.
ISSN:0098-4590