ELICITATION OF DELAYED HYPERSENSITIVITY BY SUPERNATANT FRACTIONS

Supernatant fractions obtained by mild acid hydrolysis of yeast phase Histoplasma capsulatum cell walls were active in eliciting delayed hypersensitivity skin reactions in H. capsulatum sensitized guinea pigs. Two peaks of activity occurred. The first or early activity was recoverable from the super...

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Bibliographic Details
Published in:Florida scientist 1978-10, Vol.41 (4), p.229-233
Main Authors: Sweeney, Michael J., White, Roseann S., Rosenberg, Bonita S., Calcagno, John V., Oels, Helen C.
Format: Article
Language:English
Subjects:
Acid hydrolysis
Antibodies
ANTIGENIC FRACTIONS OBTAINED FROM MILD ACID HYDROLYSIS OF YEAST PHASE HISTOPLASMA CAPSULATUM CELL WALLS, I-III
Antigens
Cell walls
Delayed hypersensitivity
Histoplasma
Hydrolysis
Precipitins
Reactivity
Yeasts
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Summary:Supernatant fractions obtained by mild acid hydrolysis of yeast phase Histoplasma capsulatum cell walls were active in eliciting delayed hypersensitivity skin reactions in H. capsulatum sensitized guinea pigs. Two peaks of activity occurred. The first or early activity was recoverable from the supernates almost immediately and persisted for 7 hr. The second or later activity became apparent at 15 hr and persisted through 48 hr of hydrolysis. The majority of the early activity was found to be released in the first 2 hr, while the later activity was released continuously between 15 and 48 hr. At least a portion of the material exhibiting the early activity was acid-stable. Skin-reactive materials were shown to be different from those involved in passive hemagglutination and fluorescence of the cell wall, as well as from those involved in precipitin reactions.
ISSN:0098-4590