Intracellular Ca2+ and not the extracellular matrix determines surface dynamics of AMPA-type glutamate receptors on aspiny neurons

The perisynaptic extracellular matrix (ECM) contributes to the control of the lateral mobility of AMPA-type glutamate receptors (AMPARs) at spine synapses of principal hippocampal neurons. Here, we have studied the effect of the ECM on the lateral mobility of AMPARs at shaft synapses of aspiny inter...

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Bibliographic Details
Published in:Philosophical transactions of the Royal Society of London. Series B. Biological sciences 2014-10, Vol.369 (1654), p.1-7
Main Authors: Klueva, Julia, Gundelfinger, Eckart D., Frischknecht, R. Renato, Heine, Martin
Format: Article
Language:English
Subjects:
AMPA receptors
Animals
Calcium - metabolism
Egtazic Acid - analogs & derivatives
Embryo, Mammalian - cytology
Extracellular Matrix
Extracellular Matrix - metabolism
Fluorescence Recovery After Photobleaching
Glutamate Receptor
Hippocampus - cytology
Interneurons
Inurement
Materials
Neurons
Neurons - metabolism
Neuroscience
Patch-Clamp Techniques
Rats
Receptors
Receptors, AMPA - metabolism
Single Particle Tracking
Synapses
Trajectories
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Summary:The perisynaptic extracellular matrix (ECM) contributes to the control of the lateral mobility of AMPA-type glutamate receptors (AMPARs) at spine synapses of principal hippocampal neurons. Here, we have studied the effect of the ECM on the lateral mobility of AMPARs at shaft synapses of aspiny interneurons. Single particle tracking experiments revealed that the removal of the hyaluronan-based ECM with hyaluronidase does not affect lateral receptor mobility on the timescale of seconds. Similarly, cross-linking with specific antibodies against the extracellular domain of the GluA1 receptor subunit, which affects lateral receptor mobility on spiny neurons, does not influence receptor mobility on aspiny neurons. AMPARs on aspiny interneurons are characterized by strong inward rectification indicating a significant fraction of Ca2+-permeable receptors. Therefore, we tested whether Ca2+ controls AMPAR mobility in these neurons. Application of the membrane-permeable Ca2+ chelator BAPTA-AM significantly increased the lateral mobility of GluA1-containing synaptic and extrasynaptic receptors. These data indicate that the perisynaptic ECM affects the lateral mobility differently on spiny and aspiny neurons. Although ECM structures on interneurons appear much more prominent, their influence on AMPAR mobility seems to be negligible at short timescales.
ISSN:0962-8436
1471-2970
DOI:10.1098/rstb.2013.0605