TRPV6 is not required for 1α,25-dihydroxyvitamin D₃-induced intestinal calcium absorption in vivo

The requirement for TRPV6 for vitamin D-dependent intestinal calcium absorption in vivo has been examined by using vitamin D-deficient TRPV6 null mice and littermate wild-type mice. Each of the vitamin D-deficient animals received each day for 4 days 50 ng of 1,25-dihydroyvitamin D₃ in 0.1 ml of 95%...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2008-12, Vol.105 (50), p.19655-19659
Main Authors: Kutuzova, Galina D, Sundersingh, Flora, Vaughan, Jennifer, Tadi, Bulli Padmaja, Ansay, Susan E, Christakos, Sylvia, DeLuca, Hector F
Format: Article
Language:English
Subjects:
1alpha,25-dichydroxycholecalciferol
1alpha,25-dihydroxyvitamin D3
Biological Sciences
blood chemistry
Calcium
Calcium channels
cholecalciferol
Diet
duodenum
Ethanol
gene expression
Gene expression regulation
intestinal absorption
Intestines
mice
microarray technology
nutrient availability
Propylene
Radioactive decay
small intestine
Vehicles
Vitamin D
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The requirement for TRPV6 for vitamin D-dependent intestinal calcium absorption in vivo has been examined by using vitamin D-deficient TRPV6 null mice and littermate wild-type mice. Each of the vitamin D-deficient animals received each day for 4 days 50 ng of 1,25-dihydroyvitamin D₃ in 0.1 ml of 95% propylene glycol:5% ethanol vehicle or vehicle only. Both the wild-type and TRPV6 null mice responded equally well to 1,25-dihydroxyvitamin D₃ in increasing intestinal calcium absorption. These results, along with our microarray data, demonstrate that TRPV6 is not required for vitamin D-induced intestinal calcium absorption and may not carry out a significant role in this process. These and previous results using calbindin D9k null mutant mice illustrate that molecular events in the intestinal calcium absorption process in response to the active form of vitamin D remain to be defined.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0810761105