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Involvement of PpDof1 transcriptional repressor in the nutrient condition-dependent growth control of protonemal filaments inPhyscomitrella patens

In higher plants, the Dof transcription factors that harbour a conserved plant-specific DNA-binding domain function in the regulation of diverse biological processes that are unique to plants. Although these factors are present in both higher and lower plants, they have not yet been characterized in...

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Published in:Journal of experimental botany 2012-01, Vol.63 (8), p.3185-3197
Main Authors: Sugiyama, Takumi, Ishida, Tetsuya, Tabei, Nobumitsu, Shigyo, Mikao, Konishi, Mineko, Yoneyama, Tadakatsu, Yanagisawa, Shuichi
Format: Article
Language:English
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Summary:In higher plants, the Dof transcription factors that harbour a conserved plant-specific DNA-binding domain function in the regulation of diverse biological processes that are unique to plants. Although these factors are present in both higher and lower plants, they have not yet been characterized in lower plants. Here six genes encoding Dof transcription factors in the mossPhyscomitrella patensare characterized and two of these genes,PpDof1andPpDof2, are functionally analysed. The targeted disruption ofPpDof1caused delayed or reduced gametophore formation, accompanied by an effect on development of the caulonema from the chloronema. Furthermore, theppdof1disruptants were found to form smaller colonies with a reduced frequency of branching of protonemal filaments, depending on the nutrients in the media. Most of these phenotypes were not apparent in theppdof2disruptant, although theppdof2disruptants also formed smaller colonies on a particular medium. Transcriptional repressor activity of PpDof1 and PpDof2 and modified expression of a number of genes in theppdofdisruptant lines were also shown. These results thus suggest that the PpDof1 transcriptional repressor has a role in controlling nutrient-dependent filament growth.
ISSN:0022-0957
1460-2431