Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient

The translocase of the mitochondrial outer membrane (TOM) complex is the main import pore for nuclear-encoded proteins into mitochondria, yet little is known about its spatial distribution within the outer membrane. Super-resolution stimulated emission depletion microscopy was used to determine quan...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2011-08, Vol.108 (33), p.13546-13551
Main Authors: Wurm, Christian A, Neumann, Daniel, Lauterbach, Marcel A, Harke, Benjamin, Egner, Alexander, Hell, Stefan W, Jakobs, Stefan
Format: Article
Language:English
Subjects:
Animals
Antiserum
Biological Sciences
Cell growth
Cell Line
Cell lines
Cell nucleus
Cell Physiological Phenomena
Correlation analysis
Fluorescence
HeLa cells
Image analysis
membrane potential
Membranes
Microscopy
Mitochondria
Mitochondria - metabolism
mitochondrial membrane
Mitochondrial Membrane Transport Proteins - analysis
Mitochondrial Membrane Transport Proteins - metabolism
Mitochondrial Membranes - metabolism
Mitochondrial Proteins - analysis
Mitochondrial Proteins - metabolism
nuclear-encoded proteins
Proteins
Rats
Receptors, Cytoplasmic and Nuclear - analysis
Receptors, Cytoplasmic and Nuclear - metabolism
Statistical variance
Tubulin - analysis
Tubulin - metabolism
Vero cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The translocase of the mitochondrial outer membrane (TOM) complex is the main import pore for nuclear-encoded proteins into mitochondria, yet little is known about its spatial distribution within the outer membrane. Super-resolution stimulated emission depletion microscopy was used to determine quantitatively the nanoscale distribution of Tom20, a subunit of the TOM complex, in more than 1,000 cells. We demonstrate that Tom20 is located in clusters whose nanoscale distribution is finely adjusted to the cellular growth conditions as well as to the specific position of a cell within a microcolony. The density of the clusters correlates to the mitochondrial membrane potential. The distributions of clusters of Tom20 and of Tom22 follow an inner-cellular gradient from the perinuclear to the peripheral mitochondria. We conclude that the nanoscale distribution of the TOM complex is finely adjusted to the cellular conditions, resulting in distribution gradients both within single cells and between adjacent cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1107553108