A Recognition Site on Synthetic Helical Oligonucleotides for Monoclonal Anti-Native DNA Autoantibody

The binding site in native DNA for a murine monoclonal anti-DNA autoantibody was investigated by measurements of competitive binding of a series of synthetic helical oligonucleotides. The antibody bound to a (dG-dC)3 or (dG-dC)4 core in the center of a base-paired octadecanucleotide. Reactions of an...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-06, Vol.83 (12), p.4469-4473
Main Authors: Stollar, B. David, Zon, Gerald, Pastor, Richard W.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, immunoglobulins
Antibodies, Monoclonal - immunology
Antibody Specificity
Atoms
Autoantibodies
Autoantibodies - immunology
Base Sequence
Binding sites
Biological and medical sciences
Chemical bases
DNA
DNA - immunology
Epitopes
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hypoxia
Lupus Erythematosus, Systemic - immunology
Mice
Models, Molecular
Molecular immunology
Monoclonal antibodies
Nucleic Acid Conformation
Nucleic acids
Oligonucleotides
Polymers
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The binding site in native DNA for a murine monoclonal anti-DNA autoantibody was investigated by measurements of competitive binding of a series of synthetic helical oligonucleotides. The antibody bound to a (dG-dC)3 or (dG-dC)4 core in the center of a base-paired octadecanucleotide. Reactions of analogues containing modifications or substitutions at specific sites indicated that the antibody antibody bound to portions of cytosine and guanine in the major groove, a limited region of the backbone, and the 2-amino group of one guanine in the minor groove. For these interactions to occur, the antibody combining site would straddle the backbone of one of the helical strands of DNA.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.12.4469