Direct Stimulation of the Guanine Nucleotide Exchange Activity of p115 RhoGEF by G$\alpha_{13}

Signaling pathways that link extracellular factors to activation of the monomeric guanosine triphosphatase (GTPase) Rho control cytoskeletal rearrangements and cell growth. Heterotrimeric guanine nucleotide-binding proteins (G proteins) participate in several of these pathways, although their mechan...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1998-06, Vol.280 (5372), p.2112-2114
Main Authors: Hart, Matthew J., Jiang, Xuejun, Kozasa, Tohru, Roscoe, William, Singer, William D., Gilman, Alfred G., Sternweis, Paul C., Bollag, Gideon
Format: Article
Language:English
Subjects:
Antiserum
COS cells
Drug interactions
Epitopes
Guanine nucleotides
Nucleotides
Physiological regulation
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Summary:Signaling pathways that link extracellular factors to activation of the monomeric guanosine triphosphatase (GTPase) Rho control cytoskeletal rearrangements and cell growth. Heterotrimeric guanine nucleotide-binding proteins (G proteins) participate in several of these pathways, although their mechanisms are unclear. The GTPase activities of two G protein α subunits, G$\alpha_{12}$ and G$\alpha_{13}$, are stimulated by the Rho guanine nucleotide exchange factor p115 RhoGEF. Activated G$\alpha_{13}$ bound tightly to p115 RhoGEF and stimulated its capacity to catalyze nucleotide exchange on Rho. In contrast, activated G$\alpha_{12}$ inhibited stimulation by G$\alpha_{13}$. Thus, p115 RhoGEF can directly link heterotrimeric G protein α subunits to regulation of Rho.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.280.5372.2112