Expression and Secretion of Type β Transforming Growth Factor by Activated Human Macrophages

Alveolar macrophages activated with concanavalin A and peripheral blood monocytes activated with lipopolysaccharide secrete type β transforming growth factor (TGF-β ). There is minimal TGF-β secretion in unactivated monocytes, even though TGF-β mRNA is expressed in these cells at a level similar to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (17), p.6020-6024
Main Authors: Assoian, Richard K., Fleurdelys, Barbara E., Stevenson, Henry C., Miller, Paul J., Madtes, David K., Raines, Elaine W., Ross, Russell, Sporn, Michael B.
Format: Article
Language:English
Subjects:
Alveolar macrophages
Biological and medical sciences
Carrier proteins
Cell growth
Cell Line
Cell lines
Cultured cells
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
In Vitro Techniques
Macrophage Activation
Macrophages
Macrophages - metabolism
Messenger RNA
Monocytes
Monocytes - metabolism
Myeloid cells: ontogeny, maturation, markers, receptors
Peptides - metabolism
Receptors, Cell Surface - metabolism
Receptors, Transforming Growth Factor beta
RNA
RNA, Messenger - metabolism
Secretion
Transforming Growth Factors
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Summary:Alveolar macrophages activated with concanavalin A and peripheral blood monocytes activated with lipopolysaccharide secrete type β transforming growth factor (TGF-β ). There is minimal TGF-β secretion in unactivated monocytes, even though TGF-β mRNA is expressed in these cells at a level similar to that in activated, lipopolysaccharidetreated cultures. U937 lymphoma cells, which have monocytic characteristics, also express mRNA for TGF-β . Freshly isolated monocytes, both control and lipopolysaccharide-treated, secrete an acid-labile binding protein that inhibits TGF-β action. We conclude the following: (i) that expression of TGF-β mRNA is unrelated to monocyte activation, (ii) that secretion of TGF-β is induced by monocyte activation, and (iii) that cosecretion of TGF-β and its monocyte/macrophage-derived binding protein may modulate growth factor action. In contrast, monocytic expression of other growth factor genes, such as the B chain of platelet-derived growth factor, is not constitutive and requires activation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.17.6020