An Early Role for Sonic Hedgehog from Foregut Endoderm in Jaw Development: Ensuring Neural Crest Cell Survival

• Published in: Proceedings of the National Academy of Sciences - PNAS 2006-08, Vol.103 (31), p.11607-11612
• Main Authors: Brito, José M., Teillet, Marie-Aimée, Le Douarin, Nicole M.
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Beak; Biological Sciences; Body Patterning; Branchial Region - anatomy & histology; Branchial Region - embryology; Cartilage; Cell adhesion & migration; Cell death; Cell Movement - physiology; Cell Survival; Cells; Chick Embryo - anatomy & histology; Chick Embryo - embryology; Chick Embryo - surgery; Digestive System - anatomy & histology; Digestive System - embryology; Ectoderm; Embryos; Endoderm; Face - anatomy & histology; Face - embryology; Foregut; Gene Expression Regulation, Developmental; Hedgehog Proteins; In Situ Hybridization; Jaw; Jaw - anatomy & histology; Jaw - embryology; Neural crest; Neural Crest - cytology; Neural Crest - physiology; Poultry; Signal Transduction - physiology; Skeleton; Trans-Activators - genetics; Trans-Activators - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0604751103

We have investigated the role of Sonic hedgehog (Shh) in the development of facial structures by depriving chicken embryos of the most anterior sources of this morphogen, including the prechordal plate and the anterior ventral endoderm of the foregut, before the onset of neural crest cell (NCC) migration to the first branchial arch (BA1). The entire forehead, including the foregut endoderm, was removed at 5- to 10-somite stage (ss), which led to the absence of the lower jaw when the operation was performed before 7-ss. If the embryos were deprived of their forehead at 8to 10-ss, they were later on endowed with a lower beak. In embryos that were operated on early, the NCCs migrated normally to BA1 but were subjected to massive apoptosis a few hours later. Cell death did not occur when forehead excision was performed at a later stage. In this case, onward expression of Shh in the ventral foregut endoderm extended caudally over the excision limit, and we hypothesized that absence of Shh production by the endoderm in embryos that were operated on early could be responsible for the NCC apoptosis and the failure of BA1 development. We thus provided exogenous Shh to the embryos that were operated on before 7-ss. In this case, the development of the lower jaw was rescued. Therefore, Shh derived from the ventral foregut endoderm ensures the survival of NCCs at a critical stage of BA1 development.