Two Human Homeobox Genes, c1 and c8: Structure Analysis and Expression in Embryonic Development

Two human cDNA clones (HHO.c1.95 and HHO.c8.5111) containing a homeobox region have been characterized, and the respective genomic regions have been partially analyzed. Expression of the corresponding genes, termed c1 and c8, was evaluated in different organs and body parts during human embryonic/fe...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1987-07, Vol.84 (14), p.4914-4918
Main Authors: Simeone, Antonio, Mavilio, Fulvio, Acampora, Dario, Giampaolo, Adele, Faiella, Antonio, Zappavigna, Vincenzo, D'Esposito, Maurizio, Pannese, Maria, Russo, Giovanni, Boncinelli, Edoardo, Peschle, Cesare
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Complementary DNA
DNA - genetics
DNA, Recombinant
Embryo, Mammalian - analysis
Embryonic and Fetal Development
Embryos
Fetus - analysis
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genes, Homeobox
Genes. Genome
Gestational Age
Homeobox genes
Human organs
Humans
Molecular and cellular biology
Molecular genetics
Oars
Poly A - analysis
RNA
RNA, Messenger - analysis
Skin
Spinal cord
Spine
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Summary:Two human cDNA clones (HHO.c1.95 and HHO.c8.5111) containing a homeobox region have been characterized, and the respective genomic regions have been partially analyzed. Expression of the corresponding genes, termed c1 and c8, was evaluated in different organs and body parts during human embryonic/fetal development. HHO.c1.95 apparently encodes a 217-amino acid protein containing a class I homeodomain that shares 60 out of 61 amino acid residues with the Antennapedia homeodomain of Drosophila melanogaster. HHO.c8.5111 encodes a 153-amino acid protein containing a homeodomain identical to that of the frog AC1 gene. Clones HHO.c1 and HHO.c8 detect by blot-hybridization one and two specific polyadenylylated transcripts, respectively. These are differentially expressed in spinal cord, backbone rudiments, limb buds (or limbs), heart, and skin of human embros and early fetuses in the 5- to 9-week postfertilization period, thus suggesting that the c1 and c8 genes play a key role in a variety of developmental processes. Together, the results of the embryonic/fetal expression of c1 and c8 and those of two previously analyzed genes (c10 and c13) indicate a coherent pattern of expression of these genes in early human ontogeny.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.14.4914