Identification of a Potent Antidiuretic Factor Acting on Beetle Malpighian Tubules

Beetles, like other insects, depend on diuretic and antidiuretic hormones to control water balance. We have isolated, using head extracts from the beetle Tenebrio molitor, a peptide that strongly inhibits fluid secretion by the Malpighian tubules of this insect. This antidiuretic factor (ADF) appear...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-01, Vol.99 (1), p.84-89
Main Authors: Eigenheer, Richard A., Nicolson, Susan W., Schegg, Kathleen M., Hull, J. Joe, Schooley, David A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Beetles
Biological Assay
Biological Sciences
Biology
Circulatory system
Corticotropin-Releasing Hormone - chemistry
Corticotropin-Releasing Hormone - pharmacology
Cyclic AMP - metabolism
Cyclic GMP - metabolism
Diuresis
Dose-Response Relationship, Drug
Drosophila melanogaster
Endothelins
Extraction
Hematophagous insects
Hormones
Insect biochemistry
Insect Hormones - chemistry
Insect Hormones - pharmacology
Insects
Malpighian tubules
Malpighian Tubules - drug effects
Molecular Sequence Data
Neurons - metabolism
Nitric Oxide - metabolism
Nitric Oxide Donors - pharmacology
Nitric Oxide Synthase - antagonists & inhibitors
Peptides - chemistry
Protein Binding
Proteins
Pupae
Second messengers
Secretion
Sequence Homology, Amino Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Studies
Tenebrio - metabolism
Tenebrio molitor
Tenebrionidae
Time Factors
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Summary:Beetles, like other insects, depend on diuretic and antidiuretic hormones to control water balance. We have isolated, using head extracts from the beetle Tenebrio molitor, a peptide that strongly inhibits fluid secretion by the Malpighian tubules of this insect. This antidiuretic factor (ADF) appears to elicit its effect via cGMP as a second messenger but does not stimulate NO production. It has primary structure: Val-Val-Asn-Thr-Pro-Gly-His-Ala-Val-Ser-Tyr-His-Val-Tyr-OH. The ADF inhibits tubule secretion with high potency: the EC50 is around 10 fM. It bears no significant resemblance to other biologically active neuropeptides. To our knowledge this is the only endogenous insect ADF acting on Malpighian tubules to be sequenced, and the first coleopteran (beetle) antidiuretic factor fully characterized to date.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.012436199