BIR-1, a Caenorhabditis elegans Homologue of Survivin, Regulates Transcription and Development

bir-1, a Caenorhabditis elegans inhibitor-of-apoptosis gene homologous to Survivin is organized in an operon with the transcription cofactor C. elegans SKIP (skp-1). Because genes arranged in operons are frequently linked functionally, we have asked whether BIR-1 also functions in transcription, bir...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2003-04, Vol.100 (9), p.5240-5245
Main Authors: Kostrouchova, Marta, Kostrouch, Zdenek, Saudek, Vladimir, Piatigorsky, Joram, Rall, Joseph Edward
Format: Article
Language:English
Subjects:
Acetylation
Animals
Antibodies
Apoptosis - physiology
Biological Sciences
Blotting, Western
Caenorhabditis elegans - embryology
Caenorhabditis elegans - genetics
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - physiology
Cell division
Cell Division - physiology
Cell Line
Cell lines
Embryos
Gene Expression Regulation, Developmental - physiology
Genes
Histones
Histones - metabolism
Hormones
Humans
Immunohistochemistry
Larvae
Larval development
Phenotypes
Ribonucleic acid
RNA
RNA Interference
Thyroid Hormones - physiology
Transcription, Genetic - physiology
Transcriptional regulatory elements
Transgenes
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Summary:bir-1, a Caenorhabditis elegans inhibitor-of-apoptosis gene homologous to Survivin is organized in an operon with the transcription cofactor C. elegans SKIP (skp-1). Because genes arranged in operons are frequently linked functionally, we have asked whether BIR-1 also functions in transcription, bir-1 inhibition resulted in multiple developmental defects that overlapped with C. elegans SKIP loss-of-function phenotypes: retention of eggs, dumpy, movement defects, and lethality. bir-1 RNA-mediated interference decreased expression of several gfp transgenes and the endogenous genes dpy-7 and hlh-1. Immunoblot analysis revealed decreased phosphoacetylated histones in bir-1 RNA-mediated interference-treated worms. In a heterologous transfection system, BIR-1 augments thyroid hormone-regulated transcription and has an additive effect with SKIP. These results show that BIR-1 functions in the regulation of transcription and development.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0730770100