A Simple ``Neural Induction'' Model with Two Interacting Cleavage-Arrested Ascidian Blastomeres

A single anterior-animal blastomere, which includes the presumptive neural region in the eight-cell embryo of the Halocynthia, a protochordate, when dissociated, cleavage-arrested with cytochalasin B, and cultured in isolation, differentiated exclusively to epidermal type judging from membrane excit...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85 (16), p.6197-6201
Main Authors: Okado, Haruo, Takahashi, Kunitaro
Format: Article
Language:English
Subjects:
Action potentials
Animals
Biological and medical sciences
Blastomeres
Blastomeres - physiology
Cell Communication
Cell Differentiation
cell interactions
Cells, Cultured
Cytochalasins
differentiation
Early stages. Segmentation. Gastrulation. Neurulation
Electric current
Electric potential
embryology
Embryology: invertebrates and vertebrates. Teratology
Embryonic Induction
Embryos
Fundamental and applied biological sciences. Psychology
Halocynthia
Immunohistochemistry
Ion Channels - physiology
Larvae
Models, Biological
P branes
Sea water
Sodium - metabolism
Tadpoles
Urochordata
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Summary:A single anterior-animal blastomere, which includes the presumptive neural region in the eight-cell embryo of the Halocynthia, a protochordate, when dissociated, cleavage-arrested with cytochalasin B, and cultured in isolation, differentiated exclusively to epidermal type judging from membrane excitability and immunoreactivity. However, when the same blastomere was cultured in contact with a single anterior-vegetal blastomere, which includes the presumptive notochordal region, it displayed Na spikes and showed no expression of the epidermal antigen, suggesting that ``neural induction'' resulted in a single cell during the interaction with a single neighboring cell. This simple two-cell system can be used for further studies on the induction mechanism.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.16.6197