Predicting the Orientation of Eukaryotic Membrane-Spanning Proteins

We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determines its orientation,...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-08, Vol.86 (15), p.5786-5790
Main Authors: Hartmann, Enno, Rapoport, Tom A., Lodish, Harvey F.
Format: Article
Language:English
Subjects:
Amino acids
Animals
Biochemistry
Biological and medical sciences
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Cytochromes
Endoplasmic Reticulum - metabolism
Facilitative glucose transport proteins
Fundamental and applied biological sciences. Psychology
Humans
mathematical models
Membrane Proteins - biosynthesis
Membrane Proteins - ultrastructure
Models, Theoretical
Molecular and cellular biology
Opsins
P branes
Protein Conformation
Protein Sorting Signals - biosynthesis
Proteins
Receptors
Structure-Activity Relationship
Topology
Ungulates
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determines its orientation, with the more positive portion facing the cytosol. In proteins that span the membrane more than once, the orientation of all subsequent transmembrane segments would be determined by that of the most N-terminal one.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.15.5786