Cloning of the cDNA and Gene for a Human D2 Dopamine Receptor

• Published in: Proceedings of the National Academy of Sciences - PNAS 1989-12, Vol.86 (24), p.9762-9766
• Main Authors: Grandy, David K., Marchionni, Mark A., Makam, Haripriya, Stofko, Renata E., Alfano, Mario, Frothingham, Leslie, Fischer, James B., Burke-Howie, Kathleen J., Bunzow, James R., Server, Alfred C., Civelli, Olivier
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acids; Animals; Base Sequence; Binding, Competitive; Biochemistry; Biological and medical sciences; Blotting, Southern; Cloning, Molecular; Complementary DNA; Corpus Striatum - metabolism; DNA; DNA - genetics; Domperidone - metabolism; Dopamine receptors; Exons; Fundamental and applied biological sciences. Psychology; Gene Library; Genes; Genes. Genome; Genomics; Humans; Introns; Kinetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nucleotide sequences; Pituitary Gland - metabolism; Rats; Receptors; Receptors, Dopamine - genetics; Receptors, Dopamine - metabolism; Receptors, Dopamine D2; Restriction Mapping; Sequence Homology, Nucleic Acid
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.86.24.9762

A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.