Antimutagenicity of WR-1065 in L5178Y Cells Exposed to Accelerated ^{56}{\rm Fe}$ Ions

• Published in: Radiation research 2002-07, Vol.158 (1), p.110-114
• Main Authors: Evans, H. H., Evans, T. E., Horng, M. F.
• Format: Article
• Language: English
• Subjects: Cell lines; CHO cells; DNA; DNA damage; Irradiation; Mutagenicity; Radiation damage; Radiation dosage; Radiation effects; Radiation protection; Short Communication
• ISSN: 0033-7587; 1938-5404

The ability of the aminothiol WR-1065 [N-(2-mercaptoethyl)-1,3-diaminopropane] to protect L5178Y (LY) cells against the cytotoxic and mutagenic effects of exposure to accelerated ^{56}{\rm Fe}$ ions (1.08 GeV/nucleon) was determined. It was found that while WR-1065 reduced the mutagenicity in both cell lines when it was present during the irradiation, the addition of WR-1065 after the exposure had no effect on the mutagenicity of the radiation in either cell line. No marked protection against the cytotoxic effects of exposure to ^{56}{\rm Fe}$ ions was provided by WR-1065 when added either during or after irradiation in either cell line. We reported previously that WR-1065 protected the LY-S1 and LY-SR1 cell lines against both the cytotoxicity and mutagenicity of X radiation when present during exposure, but that its protection when administered after exposure was limited to the mutagenic effects in the radiation-hypersensitive cell line, LY-S1. The results indicate that the mechanisms involved differ in the protection against cytotoxic compared to mutagenic effects and in the protection against damage caused by accelerated ^{56}{\rm Fe}$ ions compared to X radiation.