Regulation of Meiotic Chromatin Loop Size by Chromosomal Position

At meiotic prophase, chromatin loops around a proteinaceous core, with the sizes of these loops varying between species. Comparison of the morphology of sequence-related inserts at different sites in transgenic mice demonstrates that loop size also varies with chromosomal geography. Similarly, chrom...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-04, Vol.93 (7), p.2795-2800
Main Authors: Henry H. Q. Heng, Chamberlain, John W., Shi, Xiao-Mei, Spyropoulos, Barbara, Tsui, Lap-Chee, Moens, Peter B.
Format: Article
Language:English
Subjects:
Animals
Chromatin
Chromatin - ultrastructure
Chromosomes
Chromosomes - physiology
Chromosomes - ultrastructure
Cricetinae
DNA
Fishing lines
Fluorescence in situ hybridization
Genetics
HLA-B7 Antigen - biosynthesis
HLA-B7 Antigen - genetics
Humans
In Situ Hybridization, Fluorescence
Lymphocytes - cytology
Lymphocytes - physiology
Lymphocytes - ultrastructure
Meiosis
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Transgenic
Mitosis
Packaging
Rats
Telomeres
Transgenic animals
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Description
Summary:At meiotic prophase, chromatin loops around a proteinaceous core, with the sizes of these loops varying between species. Comparison of the morphology of sequence-related inserts at different sites in transgenic mice demonstrates that loop size also varies with chromosomal geography. Similarly, chromatin loop lengths differ dramatically for interstitially and terminally located hamster telomeric sequences. Sequences, telomeric or otherwise, located at chromosome termini, closely associate with the meiotic proteinaceous core, forming shorter loops than identical interstitial sequences. Thus, we present evidence that different chromatin packaging mechanisms exist for interstitial versus terminal chromosomal regions, which act separately from those operating at the level of the DNA sequence. Chromosomal position plays the dominant role in chromatin packaging.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.7.2795