Mutational neighbourhood and mutation supply rate constrain adaptation in Pseudomonas aeruginosa

Understanding adaptation by natural selection requires understanding the genetic factors that determine which beneficial mutations are available for selection. Here, using experimental evolution of rifampicin-resistant Pseudomonas aeruginosa, we show that different genotypes vary in their capacity f...

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Bibliographic Details
Published in:Proceedings of the Royal Society. B, Biological sciences Biological sciences, 2010-02, Vol.277 (1681), p.643-650
Main Authors: Hall, Alex R., Griffiths, Victoria F., MacLean, R. Craig, Colegrave, Nick
Format: Article
Language:English
Subjects:
Adaptation, Biological - genetics
Amino acids
Bacteria
Bacterial Proteins - genetics
Base Sequence
Biological adaptation
Compensatory Adaptation
Directed Molecular Evolution
DNA Primers - genetics
Drug Resistance, Microbial - genetics
Ecological competition
Evolution
Evolutionary genetics
Experimental Evolution
Genetic mutation
Genotype
Genotypes
Molecular Sequence Data
Mutation - genetics
Mutational Neighbourhood
Population genetics
Population size
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - physiology
Rifampin
Selection, Genetic
Sequence Analysis, DNA
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Summary:Understanding adaptation by natural selection requires understanding the genetic factors that determine which beneficial mutations are available for selection. Here, using experimental evolution of rifampicin-resistant Pseudomonas aeruginosa, we show that different genotypes vary in their capacity for adaptation to the cost of antibiotic resistance. We then use sequence data to show that the beneficial mutations associated with fitness recovery were specific to particular genetic backgrounds, suggesting that genotypes had access to different sets of beneficial mutations. When we manipulated the supply rate of beneficial mutations, by altering effective population size during evolution, we found that it constrained adaptation in some selection lines by restricting access to rare beneficial mutations, but that the effect varied among the genotypes in our experiment. These results suggest that mutational neighbourhood varies even among genotypes that differ by a single amino acid change, and this determines their capacity for adaptation as well as the influence of population biology processes that alter mutation supply rate.
ISSN:0962-8452
1471-2954
1471-2945
DOI:10.1098/rspb.2009.1630