In vitro and in vivo Two-Photon Luminescence Imaging of Single Gold Nanorods

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a$cos^4$dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-11, Vol.102 (44), p.15752-15756
Main Authors: Haifeng Wang, Terry B. Huff, Daniel A. Zweifel, He, Wei, Low, Philip S., Alexander Wei, Cheng, Ji-Xin, Shen, Y. Ron
Format: Article
Language:English
Subjects:
Animals
Blood Circulation
Blood Vessels
Diagnostic Imaging - methods
Ear - blood supply
Emission spectra
Female
Fluorescence Polarization
Gold
Imaging
Laser beams
Luminescence
Luminescent Measurements - methods
Mice
Mice, Inbred BALB C
Microscopy, Confocal - methods
Molecules
Nanorods
Nanostructures
Nanotechnology
Physical Sciences
Plasmons
Scientific imaging
Signaling
Wave excitation
Wavelengths
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Summary:Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a$cos^4$dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0504892102