A Modified System for Analyzing lonizing Radiation-Induced Chromosome Abnormalities

The analysis of dicentric chromosomes in human peripheral blood lymphocytes (PBLs) by Giemsa staining is the most established method for biological dosimetry. However, this method requires a well-trained person because of the difficulty in detecting aberrations rapidly and accurately. Here, we appli...

Full description

Saved in:
Bibliographic Details
Published in:Radiation research 2012-05, Vol.177 (5), p.533-538
Main Authors: Shi, Lin, Fujioka, Kurumi, Sun, Jiying, Kinomura, Aiko, Inaba, Toshiya, Ikura, Tsuyoshi, Ohtaki, Megu, Yoshida, Mitsuaki, Kodama, Yoshiaki, Livingston, Gordon K., Kamiya, Kenji, Tashiro, Satoshi
Format: Article
Language:English
Subjects:
Analytical estimating
Centromeres
Chromosome aberrations
Chromosomes
DNA probes
Dosimetry
Fluorescence in situ hybridization
Irradiation
Radiation dosage
Ring chromosomes
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The analysis of dicentric chromosomes in human peripheral blood lymphocytes (PBLs) by Giemsa staining is the most established method for biological dosimetry. However, this method requires a well-trained person because of the difficulty in detecting aberrations rapidly and accurately. Here, we applied a fluorescence in situ hybridization (FISH) technique, using telomere and centromere peptide nucleic acid (PNA) probes, to solve the problem of biological dosimetry in radiation emergency medicine. A comparison by a well-trained observer found that FISH analysis of PBLs for the dose estimation was more accurate than the conventional Giemsa analysis, especially in samples irradiated at high doses. These results show that FISH analysis with centromeric/telomeric PNA probes could become the standard method for biological dosimetry in radiation emergency medicine.
ISSN:0033-7587
1938-5404