Morphological, cytochemical and autoradiographic studies of local lesions induced by the U5 strain of tobacco mosaic virus in Nicotiana glutinosa L

Observations of light and electron microscopy together with experiments of autoradiography after phenylalanine-³H administration, made on leaves of Nicotiana glutinosa L. experimentally infected with TMV-U5, have demonstrated that in this hypersensitive host lignification occurs in the tissues surro...

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Bibliographic Details
Published in:Rivista di patologia vegetale 1974, Vol.10 (2), p.207-218
Main Authors: Favali, M.A, Bassi, M, Conti, G.G
Format: Article
Language:English
Subjects:
Cell walls
Electron microscopy
Lesions
Lignification
Lignin
Materials
Mesophyll cells
plant anatomy
Plant cells
plant health
plant morphology
plant viruses
Tobacco mosaic virus
Tracheids
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Summary:Observations of light and electron microscopy together with experiments of autoradiography after phenylalanine-³H administration, made on leaves of Nicotiana glutinosa L. experimentally infected with TMV-U5, have demonstrated that in this hypersensitive host lignification occurs in the tissues surrounding the necrotic lesions. Lignin deposition takes place at the border of the necrotic lesions and in the lumen of the tracheids situated in their proximity. By cytochemical staining it has been shown that in the degenerated cells inside the lesions the only recognizable structures are Golgi-derived vesicles, most likely lysosomes. Su foglie di Nicotiana glutinosa L. infette da 4 giorni con il ceppo U5 del TMV sono state eseguite osservazioni al microscopio ottico ed al microscopio elettronico, ed esperimenti di autoradiografia dopo somministrazione di fenilalanina-³H. Si è così dimostrato ehe in questo ospite ipersensibile si verifica un processo di lignificazione nei tessuti che circondano ogni lesione locale necrotica. La deposizione di lignina si verifica lungo il bordo delle lesioni necrotiche e nelle tracheidi ad esse adiacenti. Saggi citochimici di colorazione hanno messo in evidenza che nelle cellule degenerate all'interno delle lesioni la sola struttura riconoscibile è costituita da vescicole derivate dal Golgi, molto probabilmente rappresentate da lisosomi.
ISSN:0035-6441