Long noncoding RNAs regulate adipogenesis

The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long n...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2013-02, Vol.110 (9), p.3387-3392
Main Authors: Sun, Lei, Goff, Loyal A., Trapnell, Cole, Alexander, Ryan, Lo, Kinyui Alice, Hacisuleyman, Ezgi, Sauvageau, Martin, Tazon-Vega, Barbara, Kelley, David R., Hendrickson, David G., Yuan, Bingbing, Kellis, Manolis, Lodish, Harvey F., Rinn, John L.
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis - genetics
Animals
Binding sites
Biological Sciences
Brown adipocytes
Cell culture
Cell lines
Gene Expression Profiling
Gene Expression Regulation
Gene Knockdown Techniques
Genes
Information Theory
Lipogenesis
Male
Mice
Oligonucleotide Array Sequence Analysis
Open Reading Frames - genetics
Phenotype
Pluripotent stem cells
Proteins
Reproducibility of Results
Ribonucleic acid
RNA
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Small interfering RNA
T cell receptors
Transcriptome - genetics
White adipocytes
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Summary:The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long noncoding RNA (lncRNA) during adipogenesis have not been explored. Here we profiled the transcriptome of primary brown and white adipocytes, preadipocytes, and cultured adipocytes and identified 175 lncRNAs that are specifically regulated during adipogenesis. Many lncRNAs are adipose-enriched, strongly induced during adipogenesis, and bound at their promoters by key transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (CEBPα). RNAi-mediated loss of function screens identified functional lncRNAs with varying impact on adipogenesis. Collectively, we have identified numerous lncRNAs that are functionally required for proper adipogenesis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1222643110