Detection of maize yellow stripe tenui-like virus by ELISA and dot-blot tests in host plants and leafhopper vector in Egypt

Antisera to the nucleoprotein of maize yellow stripe tenui-like virus (MYSV) were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected mai...

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Bibliographic Details
Published in:Phytopathologia mediterranea 1996-04, Vol.35 (1), p.19-23
Main Authors: Mahmoud, A., Thouvenel, J.-C., Abol-Ela, S.E., Sewify, G.H., Ammar, E.D.
Format: Article
Language:English
Subjects:
Antiserum
Coatings
Corn
Host plants
Leaves
Nucleoproteins
Plant antigens
Stripes
Symptoms
Viruses
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Summary:Antisera to the nucleoprotein of maize yellow stripe tenui-like virus (MYSV) were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oats, and the graminaceous weeds Bromus wildenowii, Cenchrus biflorus, Dichanthium annulatum, Digitaria sanguinalis, Echinochloa colonum, Setaria verticillata and S. viridis. In maize leaves, differences in virus titer appeared to be correlated with leaf age and with MYSV symptom-types. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers (Cicadulina chinai).
ISSN:0031-9465
1593-2095