Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea

RAPDs-fîngerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was ext...

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Bibliographic Details
Published in:Advances in horticultural science 1996-01, Vol.10 (2), p.105-107
Main Authors: Cresti, M., Linskens, H.F., Mulcahy, D.L., Bush, S., Di Stilio, V., Xu, M.Y., Vignani, R., Cimato, A.
Format: Article
Language:English
Subjects:
Acetates
Centrifugation
DNA
DNA fingerprinting
Electrophoresis
Genotypes
Liquids
Olive oil
Polymerase chain reaction
Sediments
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Summary:RAPDs-fîngerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. DNA markers profiles of amplified samples of oil and leaf extracts, obtained with primer OPA-90 from genotype Grossolana showed a high degree of similarity.
ISSN:0394-6169
1592-1573