Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea

RAPDs-fîngerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was ext...

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Published in:Advances in horticultural science 1996-01, Vol.10 (2), p.105-107
Main Authors: Cresti, M., Linskens, H.F., Mulcahy, D.L., Bush, S., Di Stilio, V., Xu, M.Y., Vignani, R., Cimato, A.
Format: Article
Language:English
Subjects:
Acetates
Centrifugation
DNA
DNA fingerprinting
Electrophoresis
Genotypes
Liquids
Olive oil
Polymerase chain reaction
Sediments
Short Notes
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container_end_page 107
container_issue 2
container_start_page 105
container_title Advances in horticultural science
container_volume 10
creator Cresti, M.
Linskens, H.F.
Mulcahy, D.L.
Bush, S.
Di Stilio, V.
Xu, M.Y.
Vignani, R.
Cimato, A.
description RAPDs-fîngerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. DNA markers profiles of amplified samples of oil and leaf extracts, obtained with primer OPA-90 from genotype Grossolana showed a high degree of similarity.
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OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. 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subjects Acetates
Centrifugation
DNA
DNA fingerprinting
Electrophoresis
Genotypes
Liquids
Olive oil
Polymerase chain reaction
Sediments
Short Notes
title Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea
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