Factors involved during in vitro culture of Calamus rotang

Roy, A. & Saha, P.K. 1997. Factors involved during in vitro culture of Calamus rotang. Calamus rotang is a subterranean woody monocot where in vitro multiplication technique may be used as an alternative process for rapid plant generation. Since subterrestrial young plantlets face a problem in e...

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Published in:Journal of tropical forest science 1997-12, Vol.10 (2), p.225-232
Main Authors: Roy, A, Saha, P.K. (Bose Institute, 93/1, A.P.C. Road, Calcutta 700 009, (India). Dept. of Botany)
Format: Article
Language:English
Subjects:
ARBUSTE
ARBUSTOS
BOSQUE TROPICAL HUMEDO
CALAMUS (PALMAE)
Chemical sterilization
Chlorides
CULTIVO IN VITRO
CULTURE IN VITRO
ESTERILIZACION (REPRODUCCION)
FORET TROPICALE HUMIDE
Hypochlorite
IN VITRO CULTURE
MULTIPLICATION DES PLANTES
Plant growth
PLANT PROPAGATION
Plant tissues
PLANTAS LENOSAS
PLANTE LIGNEUSE
Plantlets
Plants
PROPAGACION DE PLANTAS
SHRUBS
Sodium
STERILISATION
STERILIZATION
Tissue culture techniques
Tropical forests
TROPICAL RAIN FORESTS
WOODY PLANTS
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Summary:Roy, A. & Saha, P.K. 1997. Factors involved during in vitro culture of Calamus rotang. Calamus rotang is a subterranean woody monocot where in vitro multiplication technique may be used as an alternative process for rapid plant generation. Since subterrestrial young plantlets face a problem in establishment due to a high degree of contamination in the medium, to reduce contamination, chemicals were tested for sterilisation of the explant. Sodium hypochlorite (5.0 percent v/v) in combination with mercuric chloride (0.5 percent w/v) resulted in the highest response. Explant establishment was dependent on their lengths as well as the plantlets from where the explants were derived. Explants of length 1.5-2.0 cm derived from plantlets having a length of 10-20 cm showed a higher rate of growth under culture conditions. Bud proliferation in explant was achieved in the establisment medium supplemented with BAP (6-benzylaminopurine) (5-10mg per l).
ISSN:0128-1283