Fluorometric Ribof lavin Titration in Plasma by Riboflavin-Binding Apoprotein as a Method for Vitamin B₂ Status Assessment

A method for plasma riboflavin determination by riboflavin-binding apoprotein titration has been proposed for vitamin B₂ status evaluation. The method is based on the formation of riboflavin-apoprotein complex accompanied by full loss of fluorescence peculiar to free riboflavin. The data obtained ha...

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Bibliographic Details
Published in:Annals of nutrition and metabolism 1995-11, Vol.39 (6), p.355-360
Main Authors: Kodentsova, Vera M., Vrzhesinskaya, Oksana A., Spirichev, Vladimir B.
Format: Article
Language:English
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Summary:A method for plasma riboflavin determination by riboflavin-binding apoprotein titration has been proposed for vitamin B₂ status evaluation. The method is based on the formation of riboflavin-apoprotein complex accompanied by full loss of fluorescence peculiar to free riboflavin. The data obtained have demonstrated a correlation with indicators of the vitamin B₂ status such as urinary excretion, erythrocyte content, and stimulation of the glutathione reductase activity by flavin adenine dinucleotide. Levels >6 ng/ml blood plasma may be considered to be a criterion for normal vitamin B₂ supply.
ISSN:0250-6807
1421-9697