Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling

The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. One of these exclusively acylates two of the six tRNALeuspecies present in this tissue. The remaining two enzyme fractions charge the other four tRNALeuspecies equally well...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1971-05, Vol.68 (5), p.873-876
Main Authors: Kanabus, Jan, Cherry, Joe H.
Format: Article
Language:English
Subjects:
Amino acids
Biological Sciences: Biochemistry
Chromatography
Cotyledons
Enzymes
Fractionation
Freon
Glycine max
Hydrocarbons, Halogenated
Hydroxyapatites
Hypocotyls
Leucine - metabolism
Ligases - analysis
Ligases - isolation & purification
Organ Specificity
Potassium phosphates
RNA, Transfer - metabolism
Seeds - enzymology
Soybeans
Transfer RNA
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Summary:The leucyl-tRNA synthetase activity from cotyledons of 4-day-old soybean seedlings has been fractionated into three components. One of these exclusively acylates two of the six tRNALeuspecies present in this tissue. The remaining two enzyme fractions charge the other four tRNALeuspecies equally well. Soybean hypocotyls appear to contain only the last two enzyme fractions.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.68.5.873