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Mucopolysaccharide and Protein--Polysaccharide of a Transplantable Rat Chondrosarcoma
Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfa...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 1971-05, Vol.68 (5), p.877-879 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfate and chondroitin 6-sulfate were absent. Extraction of the fresh tumor gave two protein--polysaccharides of similar carbohydrate composition, one soluble in 0.5 M NaCl, the other insoluble. The latter was solubilized in 4 M guanidineĀ· HCl. A dialyzable fraction from the 4 M guanidine solution may be responsible for the insolubility. Both protein--polysaccharides were antigenic and cross-reacted with similar fractions of bovine and human cartilage. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.68.5.877 |