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Mucopolysaccharide and Protein--Polysaccharide of a Transplantable Rat Chondrosarcoma

Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfa...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1971-05, Vol.68 (5), p.877-879
Main Authors: Choi, Haing Ug, Meyer, Karl, Swarm, Richard
Format: Article
Language:English
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Summary:Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfate and chondroitin 6-sulfate were absent. Extraction of the fresh tumor gave two protein--polysaccharides of similar carbohydrate composition, one soluble in 0.5 M NaCl, the other insoluble. The latter was solubilized in 4 M guanidineĀ· HCl. A dialyzable fraction from the 4 M guanidine solution may be responsible for the insolubility. Both protein--polysaccharides were antigenic and cross-reacted with similar fractions of bovine and human cartilage.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.68.5.877