Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism

Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in eit...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-04, Vol.71 (4), p.1128-1132
Main Authors: Fridlender, Bertold R., Medrano, Estela, Mordoh, Jose
Format: Article
Language:English
Subjects:
Actinomycin
Biological Sciences: Cell Biology
Cell Fractionation
Cell nucleus
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cells
Chemical suspensions
Cycloheximide - pharmacology
Cytarabine - pharmacology
Cytoplasm - drug effects
Cytoplasm - metabolism
Dactinomycin - pharmacology
DNA
DNA - biosynthesis
DNA Nucleotidyltransferases - metabolism
DNA Replication - drug effects
Enzymes
Homogenization
Humans
Lectins - pharmacology
Lymphocytes
Lymphocytes - cytology
Lymphocytes - drug effects
Lymphocytes - enzymology
Lymphocytes - metabolism
Nucleic acid precursors
Stem cells
Stimulation, Chemical
Thymidine - metabolism
Thymine Nucleotides - metabolism
Tritium
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Summary:Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arabinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.4.1128