Localization of Escherichia coli Ribosomal Proteins S4 and S14 by Electron Microscopy of Antibody-Labeled Subunits

Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 I...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-12, Vol.71 (12), p.4688-4692
Main Authors: Lake, James A., Pendergast, Maryanne, Kahan, Lawrence, Nomura, Masayasu
Format: Article
Language:English
Subjects:
Aggregation
Antibodies
Bacterial Proteins - analysis
Bacterial Proteins - immunology
Binding Sites, Antibody
Biochemistry
Biological Sciences: Biochemistry
Dimers
Electron microscopy
Electrons
Epitopes
Escherichia coli - ultrastructure
Immunoglobulin Fab Fragments
Immunoglobulin Fc Fragments
Immunoglobulin G
Microscopy, Electron
Molecules
Monomers
Ribosomal proteins
Ribosomes
Ribosomes - analysis
Ribosomes - immunology
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Summary:Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.12.4688