Induction of Error-Prone Repair as a Consequence of DNA Ligase Deficiency in Escherichia coli

DNA ligase deficiency is shown to induce generalized mutator activity in E. coli. This mutator activity is unaffected by 3 μ g/ml of chloramphenicol but is abolished both in lig-recA double mutants and by incubation with 20 μ g/ml of chloramphenicol. DNA ligase deficiency is also shown to reactivate...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-11, Vol.72 (11), p.4645-4649
Main Authors: Morse, Lawrence S., Pauling, Crellin
Format: Article
Language:English
Subjects:
Bacteriophage T7
Bacteriophages
Chloramphenicol - pharmacology
Coliphages - radiation effects
DNA
DNA damage
DNA Repair
Dose-Response Relationship, Radiation
Escherichia coli - enzymology
Escherichia coli - radiation effects
Genetic mutation
Inductive reasoning
Irradiation
Lesions
Mutagenesis
Mutation - drug effects
Polynucleotide Ligases - metabolism
Prophages
Radiation Effects
Temperature
Ultraviolet Rays
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Summary:DNA ligase deficiency is shown to induce generalized mutator activity in E. coli. This mutator activity is unaffected by 3 μ g/ml of chloramphenicol but is abolished both in lig-recA double mutants and by incubation with 20 μ g/ml of chloramphenicol. DNA ligase deficiency is also shown to reactivate ultraviolet light-irradiated phage lambda and T7 and to increase both spontaneous and ultraviolet light-induced mutagenesis in phage lambda, all of which are abolished in lig-recA strains. Interaction occurs between the molecular events of mutagenesis induced by ultraviolet irradiation and those induced by DNA ligase deficiency. These observations suggest a common pathway, coordinately expressed with the inducible repair mode, that is responsible for mutagenesis.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.11.4645