Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts

60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their funct...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-12, Vol.72 (12), p.4744-4748
Main Authors: Moller, W., Slobin, L. I., Amons, R., Richter, D.
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Animals
Artemia
Binding Sites
Biochemistry
Cysts
Decapoda (Crustacea)
Electrophoresis
Gels
Molecular Weight
Peptide Chain Elongation, Translational
Peptide Elongation Factors
Peptide Fragments - analysis
Protein Binding
Ribosomal proteins
Ribosomal Proteins - analysis
Ribosomes
Ribosomes - chemistry
Ribosomes - metabolism
RNA, Transfer - metabolism
Sodium
Sulfates
Transfer RNA
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Summary:60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their functional requirement in the elongation factor dependent binding of aminoacyl transfer RNA to the ribosome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.12.4744