Loading…

Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts

60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their funct...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-12, Vol.72 (12), p.4744-4748
Main Authors: Moller, W., Slobin, L. I., Amons, R., Richter, D.
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c3764-5661533eafbd5a05eb6068f91add84a4744f506656759ee1cd440f22463df46d3
cites
container_end_page 4748
container_issue 12
container_start_page 4744
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 72
creator Moller, W.
Slobin, L. I.
Amons, R.
Richter, D.
description 60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their functional requirement in the elongation factor dependent binding of aminoacyl transfer RNA to the ribosome.
doi_str_mv 10.1073/pnas.72.12.4744
format article
fullrecord <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_jstor_primary_65279</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>65279</jstor_id><sourcerecordid>65279</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3764-5661533eafbd5a05eb6068f91add84a4744f506656759ee1cd440f22463df46d3</originalsourceid><addsrcrecordid>eNp9kc1vEzEQxa0K1IbQcyWkIp_gtKm_vXvgEEV8VKrUCsrZnaxt6mp3HWwHKH89uySUckGyZGne7808exA6oWRBieZnmwHyQrMFZQuhhThAM0oaWinRkCdoRgjTVS2YOELPcr4jhDSyJofokBI1nnqGbs5z7KCEOGAYLF7dQoK2uBR-7orR4-vvES_bYEOLr1IsLgx5KivyCX8M65hj7zL2KfZ4mYrrA-AMXRgAr-5zyc_RUw9ddsf7e44-v3t7vfpQXVy-P18tL6qWayUqqRSVnDvwayuBSLdWYz7fULC2FjC9zUuilFRaNs7R1gpBPGNCceuFsnyO3uz6brbr3tnWDSVBZzYp9JDuTYRg_lWGcGu-xG-G13U9_uQcvdr7U_y6dbmYPuTWdR0MLm6zqTllXGg1gmc7sE0x5-T8wwxKzLQTM-3EaGYoM1Pu0XH6ONoj_vcSRv31Xp-Mf9S_DYzfdl1xP8pIvvwvOQIvdsBdLjE9EEoy3fBfnx2pxw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83123476</pqid></control><display><type>article</type><title>Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts</title><source>JSTOR Archival Journals and Primary Sources Collection</source><source>PubMed Central</source><creator>Moller, W. ; Slobin, L. I. ; Amons, R. ; Richter, D.</creator><creatorcontrib>Moller, W. ; Slobin, L. I. ; Amons, R. ; Richter, D.</creatorcontrib><description>60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their functional requirement in the elongation factor dependent binding of aminoacyl transfer RNA to the ribosome.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.72.12.4744</identifier><identifier>PMID: 1061068</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acids - analysis ; Animals ; Artemia ; Binding Sites ; Biochemistry ; Cysts ; Decapoda (Crustacea) ; Electrophoresis ; Gels ; Molecular Weight ; Peptide Chain Elongation, Translational ; Peptide Elongation Factors ; Peptide Fragments - analysis ; Protein Binding ; Ribosomal proteins ; Ribosomal Proteins - analysis ; Ribosomes ; Ribosomes - chemistry ; Ribosomes - metabolism ; RNA, Transfer - metabolism ; Sodium ; Sulfates ; Transfer RNA</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1975-12, Vol.72 (12), p.4744-4748</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3764-5661533eafbd5a05eb6068f91add84a4744f506656759ee1cd440f22463df46d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/72/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/65279$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/65279$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1061068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moller, W.</creatorcontrib><creatorcontrib>Slobin, L. I.</creatorcontrib><creatorcontrib>Amons, R.</creatorcontrib><creatorcontrib>Richter, D.</creatorcontrib><title>Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their functional requirement in the elongation factor dependent binding of aminoacyl transfer RNA to the ribosome.</description><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Artemia</subject><subject>Binding Sites</subject><subject>Biochemistry</subject><subject>Cysts</subject><subject>Decapoda (Crustacea)</subject><subject>Electrophoresis</subject><subject>Gels</subject><subject>Molecular Weight</subject><subject>Peptide Chain Elongation, Translational</subject><subject>Peptide Elongation Factors</subject><subject>Peptide Fragments - analysis</subject><subject>Protein Binding</subject><subject>Ribosomal proteins</subject><subject>Ribosomal Proteins - analysis</subject><subject>Ribosomes</subject><subject>Ribosomes - chemistry</subject><subject>Ribosomes - metabolism</subject><subject>RNA, Transfer - metabolism</subject><subject>Sodium</subject><subject>Sulfates</subject><subject>Transfer RNA</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><recordid>eNp9kc1vEzEQxa0K1IbQcyWkIp_gtKm_vXvgEEV8VKrUCsrZnaxt6mp3HWwHKH89uySUckGyZGne7808exA6oWRBieZnmwHyQrMFZQuhhThAM0oaWinRkCdoRgjTVS2YOELPcr4jhDSyJofokBI1nnqGbs5z7KCEOGAYLF7dQoK2uBR-7orR4-vvES_bYEOLr1IsLgx5KivyCX8M65hj7zL2KfZ4mYrrA-AMXRgAr-5zyc_RUw9ddsf7e44-v3t7vfpQXVy-P18tL6qWayUqqRSVnDvwayuBSLdWYz7fULC2FjC9zUuilFRaNs7R1gpBPGNCceuFsnyO3uz6brbr3tnWDSVBZzYp9JDuTYRg_lWGcGu-xG-G13U9_uQcvdr7U_y6dbmYPuTWdR0MLm6zqTllXGg1gmc7sE0x5-T8wwxKzLQTM-3EaGYoM1Pu0XH6ONoj_vcSRv31Xp-Mf9S_DYzfdl1xP8pIvvwvOQIvdsBdLjE9EEoy3fBfnx2pxw</recordid><startdate>19751201</startdate><enddate>19751201</enddate><creator>Moller, W.</creator><creator>Slobin, L. I.</creator><creator>Amons, R.</creator><creator>Richter, D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19751201</creationdate><title>Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts</title><author>Moller, W. ; Slobin, L. I. ; Amons, R. ; Richter, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3764-5661533eafbd5a05eb6068f91add84a4744f506656759ee1cd440f22463df46d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Artemia</topic><topic>Binding Sites</topic><topic>Biochemistry</topic><topic>Cysts</topic><topic>Decapoda (Crustacea)</topic><topic>Electrophoresis</topic><topic>Gels</topic><topic>Molecular Weight</topic><topic>Peptide Chain Elongation, Translational</topic><topic>Peptide Elongation Factors</topic><topic>Peptide Fragments - analysis</topic><topic>Protein Binding</topic><topic>Ribosomal proteins</topic><topic>Ribosomal Proteins - analysis</topic><topic>Ribosomes</topic><topic>Ribosomes - chemistry</topic><topic>Ribosomes - metabolism</topic><topic>RNA, Transfer - metabolism</topic><topic>Sodium</topic><topic>Sulfates</topic><topic>Transfer RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moller, W.</creatorcontrib><creatorcontrib>Slobin, L. I.</creatorcontrib><creatorcontrib>Amons, R.</creatorcontrib><creatorcontrib>Richter, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moller, W.</au><au>Slobin, L. I.</au><au>Amons, R.</au><au>Richter, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1975-12-01</date><risdate>1975</risdate><volume>72</volume><issue>12</issue><spage>4744</spage><epage>4748</epage><pages>4744-4748</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>60S ribosomes from encysted gastrulae of the brine shrimp Artemia salina contain two acidic proteins, which are homologous to the Escherichia coli proteins L7 and L12. The proteins were purified and characterized with respect to molecular weight, amino-acid composition, peptide maps, and their functional requirement in the elongation factor dependent binding of aminoacyl transfer RNA to the ribosome.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1061068</pmid><doi>10.1073/pnas.72.12.4744</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1975-12, Vol.72 (12), p.4744-4748
issn 0027-8424
1091-6490
language eng
recordid cdi_jstor_primary_65279
source JSTOR Archival Journals and Primary Sources Collection; PubMed Central
subjects Amino Acids - analysis
Animals
Artemia
Binding Sites
Biochemistry
Cysts
Decapoda (Crustacea)
Electrophoresis
Gels
Molecular Weight
Peptide Chain Elongation, Translational
Peptide Elongation Factors
Peptide Fragments - analysis
Protein Binding
Ribosomal proteins
Ribosomal Proteins - analysis
Ribosomes
Ribosomes - chemistry
Ribosomes - metabolism
RNA, Transfer - metabolism
Sodium
Sulfates
Transfer RNA
title Isolation and Characterization of Two Acidic Proteins of 60S Ribosomes from Artemia salina Cysts
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T12%3A03%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20Characterization%20of%20Two%20Acidic%20Proteins%20of%2060S%20Ribosomes%20from%20Artemia%20salina%20Cysts&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Moller,%20W.&rft.date=1975-12-01&rft.volume=72&rft.issue=12&rft.spage=4744&rft.epage=4748&rft.pages=4744-4748&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.72.12.4744&rft_dat=%3Cjstor_pnas_%3E65279%3C/jstor_pnas_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3764-5661533eafbd5a05eb6068f91add84a4744f506656759ee1cd440f22463df46d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=83123476&rft_id=info:pmid/1061068&rft_jstor_id=65279&rfr_iscdi=true