Chemical Transmission between Rat Sympathetic Neurons and Cardiac Myocytes Developing in Microcultures: Evidence for Cholinergic, Adrenergic, and Dual-Function Neurons

Electrophysiological studies were made on microcultures (300-500 μ m in diameter) in which solitary sympathetic principal neurons from newborn rats grew on previously dissociated rat heart cells. Some neurons inhibited, some excited, and others first inhibited and then excited the cardiac myocytes....

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1976-11, Vol.73 (11), p.4225-4229
Main Authors: Furshpan, E. J., MacLeish, P. R., O'Lague, P. H., Potter, D. D.
Format: Article
Language:English
Subjects:
Acetylcholine - metabolism
Adrenergics
Animals
Atropine
Catecholamines
Cells, Cultured
Cholinergics
Cultured cells
Ganglia, Autonomic - cytology
Heart - innervation
Kinetics
Myocardium - cytology
Neurons
Neurons - metabolism
Norepinephrine - metabolism
Propranolol - pharmacology
Rats
Receptors, Nicotinic - metabolism
Secretion
Synapses
Synapses - metabolism
Synaptic transmission
Synaptic Transmission - drug effects
Transmitters
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Summary:Electrophysiological studies were made on microcultures (300-500 μ m in diameter) in which solitary sympathetic principal neurons from newborn rats grew on previously dissociated rat heart cells. Some neurons inhibited, some excited, and others first inhibited and then excited the cardiac myocytes. Application of drugs provided evidence for secretion of acetylcholine by the first group, catecholamines by the second, and both acetylcholine and catecholamines by the third. Solitary neurons which inhibited the myocytes usually excited themselves at nicotinic synapses (autapses).
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.73.11.4225