Hydrogen-Bonded Protons in the Tertiary Structure of Yeast tRNAPhein Solution

Temperature-dependent lowfield proton magnetic resonance spectra of yeast tRNAPhewere recorded between 10 and 15 parts per million. Seven resonances of hydrogen-bonded protons disappeared reversibly under two sets of conditions where the selective broadening of tertiary structure resonances was pred...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1977-04, Vol.74 (4), p.1561-1564
Main Authors: Römer, R., Varadi, V.
Format: Article
Language:English
Subjects:
Chemical equilibrium
Hydrogen bonds
Melting
Nuclear magnetic resonance
Proton magnetic resonance
Protons
Resonance
Ring currents
Transfer RNA
Yeasts
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Summary:Temperature-dependent lowfield proton magnetic resonance spectra of yeast tRNAPhewere recorded between 10 and 15 parts per million. Seven resonances of hydrogen-bonded protons disappeared reversibly under two sets of conditions where the selective broadening of tertiary structure resonances was predicted by temperature jump experiments. The seven resonances were assigned to the seven tertiary hydrogen bonds expected between 10 and 15 parts per million from the crystal structure of yeast tRNAPhe. Some of the non-Watson-Crick base pairs have unusual unshifted standard chemical shifts after the ring current contributions calculated from the crystal coordinates were subtracted. The differences of the chemical shifts of homologous tertiary structure base pairs in Escherichia coli tRNAfMetand yeast tRNAPhegive experimental evidence for details of the conformational differences postulated by model building on the basis of the x-ray coordinates of yeast tRNAPheand sequence homologies.
ISSN:0027-8424