Polypeptide Hormone Binding Sites in vivo: Initial Localization of125I-Labeled Insulin to Hepatocyte Plasmalemma as Visualized by Electron Microscope Radioautography

The rationale of the specific-binding assay was applied to the detection of the liver insulin receptor in vivo. Quantitative electron microscope radioautography indicated that, 3 min after an intraportal injection,125I-insulin was exclusively located to the hepatocyte plasmalemma.

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1977-11, Vol.74 (11), p.5051-5055
Main Authors: Bergeron, J. J. M., Levine, G., Sikstrom, R., O'Shaughnessy, D., Kopriwa, B., Nadler, N. J., Posner, B. I.
Format: Article
Language:English
Subjects:
Autoradiography
Cell membranes
Electron microscopes
Endothelial cells
Hepatocytes
Insulin
Kupffer cells
Liver
Microscopes
Sine waves
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Description
Summary:The rationale of the specific-binding assay was applied to the detection of the liver insulin receptor in vivo. Quantitative electron microscope radioautography indicated that, 3 min after an intraportal injection,125I-insulin was exclusively located to the hepatocyte plasmalemma.
ISSN:0027-8424