Ecdysteroid Receptors in Imaginal Discs of Drosophila melanogaster

[3H]Ponasterone A (PNA) of high specific activity has been used to identify and begin characterization of ecdysteroid (formerly called ecdysone) receptors in cytoplasmic and nuclear fractions of imaginal discs of Drosophila melanogaster. The equilibrium Kd of the observed macromolecular binding, 3-4...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1978-12, Vol.75 (12), p.6039-6043
Main Authors: Yund, Mary Alice, King, David S., Fristrom, James W.
Format: Article
Language:English
Subjects:
Animals
Cell lines
Cell nucleus
Cell Nucleus - metabolism
Cholestenes - metabolism
Cytoplasm - metabolism
Cytosol
Dose-Response Relationship, Drug
Drosophila
Drosophila melanogaster - anatomy & histology
Drosophila melanogaster - metabolism
Ecdysone - metabolism
Ecdysteroids
Ecdysterone - metabolism
Hormones
Imaginal discs
Kinetics
Radioactive decay
Receptors
Receptors, Cell Surface - metabolism
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Description
Summary:[3H]Ponasterone A (PNA) of high specific activity has been used to identify and begin characterization of ecdysteroid (formerly called ecdysone) receptors in cytoplasmic and nuclear fractions of imaginal discs of Drosophila melanogaster. The equilibrium Kd of the observed macromolecular binding, 3-4 × 10-9 M PNA, is in good agreement with the minimal concentration required for induction of complete morphogenesis in vitro, 4.2 × 10-9 M PNA. Binding is analog specific and has kinetics consistent with a role in hormone response. On gentle homogenization,
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.12.6039